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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has long been observed in many tumors, which ends up in chromosome instability, and so may possibly lead to tumorigenesis. This protein was identified to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle regulated histone gene expression and plays a significant function in selling mobile-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are actually described. Two extra splice variants ended up documented but specific nucleotide sequence data is not however readily available.
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Blocking peptides are peptides that bind particularly on the goal antibody and block antibody binding. These peptide commonly includes the epitope identified through the antibody.
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This manual outlines more feasible optimization of cellular and immunoassay parameters to ensure the very best success are...
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This manual outlines more possible optimization of cellular and immunoassay parameters to be certain the absolute best final click here results are...
Antibodies certain to the blocking peptide not bind for the epitope within the goal protein. By comparing the staining from the blocked antibody versus the antibody by itself, you can see which staining is distinct.
Goal Facts Cyclin E belongs to your remarkably conserved cyclin household, whose users are characterised by a spectacular periodicity in protein abundance with the cell cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins exhibit unique expression and degradation patterns which contribute into the temporal coordination of each mitotic party. Cyclin E types a posh with and capabilities to be a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells progress through S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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